Euthanasia
From SPNHC Wiki
Contents
Statement of Purpose
These links and documents contain information about best practices for euthanasia.
Introduction
Contributors
Fishes
(Note: acceptable methods depend on institutional IACUC)
- MS-222-immersion
- Directly into formalin (5-10%) (e.g., larval fishes); see subsampling below
- Directly into ethanol (70-95%)
- Freezing (ice and water bath)
Remember to conduct any live-color photography prior to euthanizing to preserve color
Herps
MS-222 (50mg/L??) immersion (amphibians) MS-222 injection (reptiles) Benzocaine (amphibians) Ethyl acetate Directly into formalin (5-10%) (e.g., larval); see subsampling below Directly into ethanol (10%) Freezing